Custom Services for Life Sciences
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Welcome to our Gene Synthesis Division.
Your knowledgeable partner for your gene synthesis projects.
Benefit from our gene synthesis team's many years experience in the fields of bioinformatics, molecular biology, and nucleic acid chemistry with unconditionally guaranteed sequences, backed up by strong QC procedures.
We offer a complete range of genetic engineering services
+ sequence design
+ subcloning in any vector
+ site-directed mutagenesis
+ gene variants design
+ large scale plasmid preparations.
pGB219 is our prefered pUC19 vector with modified restriction sites.
Alternatively, we can clone your gene into any vector of your choice.
You would need to provide us with: 5 ug of purified plasmid vector Sequence information Additional charge applies, please request a quote.
Basic Local Alignment Search Tool
BLAST finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.
With this service, we would introduce any types of mutation into your gene(s) of interest.
+ Directed single point missense mutations: transitions / transversions - conservative/non-conservative
+ Simultaneous multiple directed point mutations
+ Directed in-frame deletions or insertions
+ 5'- / 3'- in-frame troncations
+ 5'- / 3'- additions-fusions
Point missense mutations may be introduced in a random fashion within a localized region of your target ORF.
Deliverables include 5 µg of purified individual plasmid DNA and double-stand sequencing data for every variant.
Applications are numerous including:
Fusion protein design
Function and activity studies and possibly optimisation